We use tblastx (translating query to all reading frames and compare it with a nucleotide database translated to all reading frames = computing time is equivalent to 36 x blastp) to find remote relatives for virus isolates that we have found in environmental samples. A while ago I asked Daniel if tblastx could be included in Megan. Daniel already has blastx functionality. He replied that tblastx would require quite a bit of work and he never had this request before. I think that without tblastx Megan is already a very useful piece of software, and I do not want to bother Daniel with this, unless this functionality would be useful for other people.
So, please let me/Daniel know if you would like to have this functionality. If not I, am ok with it, but if you do want to use it, and if Daniel can implement, I would definitely use it.