Hi there, I’ve been using MEGAN to compare metagenomic samples between sequencing platforms and classifiers, however I cannot get the SILVA taxonomy to operate. I have been able to generate my .biom from the 16s analysis, however it contains only sample names, everything remains unassigned. I assume this is due to not having the SIVLA mapping file incorporated, however I’m not sure how to do this.
I assume I require the UE to do this analysis?
The “Use Alternative Taxonomy” option in “Edit -> Preferences” is greyed out.
Thanks a lot!