This is just to clear some confusion.
When any .biom file in imported in MEGAN, is it possible that 1 OTU can be placed in multiple places in taxonomy tree?
The reason I am asking, I recently used MEGAN for analysing some files which had been processed with QIIME and thus imported comparison biom file in megan.
For example when I import one “otu_table.biom” (generated by QIIME) file in MEGAN the total no varies.
I see total no of OTUs in the summary file that QIIME generated as:
Now after importing the .biom file in megan, when all the tree is uncollapsed I exported all taxa (only assigned reads) to a file.
Now if I make a sum of all column from this file I should get the total no of OTUs in each sample. …which is not match with what I had before.
Min OTUs 12812
Max OTUs 209881
So its good to know if 1 OTU can be place in multiple places in taxonomy tree in megan?
Thanks a lot,