Dear MEGAN users,
I download non-redundant proteins of bacteria and archaea fron NCBI refseq database released September 16, 2019 and make a diamond dmnd file.
I blast paired-end mRNA sequences against the database and I got the daa output.
When I meganized the daa file using
bash /software/megan/tools/daa-meganizer -i qc_pear_sort.daa -g2t prot_acc2tax-Jul2019X1.abin -g2eggnog acc2eggnog-Jul2019X.abin -g2interpro2go acc2interpro-Jul2019X.abin -g2seed acc2seed-May2015XX.abin.
Only reads were mapped to taxonomy database but no reads were mapped to eggnog, seed, interpro2go database.
I updated the MEGAN version from 6.17.0 to 6.18.0 and downloaded megan-map-Oct2019.db.
DAA file was meganized by the below command:
bash daa-meganizer -i IS1_qc_pear_sort.daa -mdb megan-map-Oct2019.db
But when I open the meganized file in MEGAN 6.18.0, megan was hanging and it shows
IOException: TRANSLUCENT translucency is not supported.
Does anyone have any idea why it happen and how I should fix this problem?
Thank you very much.