I would like to analyze my metagenomics data using your tools (DIAMOND and MEGAN).
I have 8 samples containing a total of ~235 million reads, at ~230bp long.
I plan to split each sample into 4 smaller chunks to reduce DIAMOND processing time.
(1) If I set the -o parameter for .m8 output, will I be able to join the 4 .m8 outputs created for each sample into one large .m8 file?
(2) If I set the -a parameter instead, will I be able to join the 4 .daa outputs for each sample into one large .daa file?
(3) Still using the -a parameter, will I be able to merge the 4 .daa outputs during the conversion to blast tabular format? i.e. will I be able to convert 4 .daa files into one blast tabular file?
(4) is there a way to join multiple DIAMOND outputs into one MEGAN input file using MEGAN?
If the answer is yes to any of these questions, could you please share how? Any help will be greatly appreciated.
Many thanks in advance,