Hello everyone. I am new at MEGAN, and might have some basic doubts that I could not figured out by reading the manual.
One of them is related to this post of Rewati27. I just uploaded 9 metagenomic samples for analyzing at MEGAN6 (version 6.0.1 for MacOS). The samples were annotated against NCBI-nr database using BLASTX. I uploaded the rma files, although, I cannot access any of the functional annotations. KEGG, SEED, EggNOG, and other functional icons (of the toolbar) are all unactivated (B&W). I also can't chose these database at the path " Change LCA parameter --> analyze using SEED; for example.
What should I do to proceed with functional annotations? Thanks