I started testing the Megan U trial with the blast file (.daa and .m8)
Now please help me rectify the problem.
- I used nucl_acc2tax-Nov2016.abin file for taxonomic mapping yet there was no taxonomic description done (see screenshot)
- Is pfam an inbuilt function as I could not find a mapping file for that on the download page, while putting the blast file there was an option for mapping file.
- KEGG and SEED had reads assigned to it but no sub classification was done in the result which is strange.(see screenshot)
- Please clarify on LCA parameters for the analysis(should it be left as it is)
Here are the parameter and mapping file I used
Taxonomy - nucl_acc2tax-Nov2016.abin
EGGNOG - acc2eggnog-Oct2016X.abin
KEGG - acc2kegg-Nov2016X-ue.abin
PFAM – “no file available on download page”
SEED - acc2seed-May2015XX.abin
here is screen shot with log file, taxonomy and KEGG window. Please help rectify the problem