I started testing the Megan U trial with the blast file (.daa and .m8)
Now please help me rectify the problem.
1) I used nucl_acc2tax-Nov2016.abin file for taxonomic mapping yet there was no taxonomic description done (see screenshot)
2) Is pfam an inbuilt function as I could not find a mapping file for that on the download page, while putting the blast file there was an option for mapping file.
3) KEGG and SEED had reads assigned to it but no sub classification was done in the result which is strange.(see screenshot)
4) Please clarify on LCA parameters for the analysis(should it be left as it is)
Here are the parameter and mapping file I used
Taxonomy - nucl_acc2tax-Nov2016.abin
EGGNOG - acc2eggnog-Oct2016X.abin
KEGG - acc2kegg-Nov2016X-ue.abin
PFAM – “no file available on download page”
SEED - acc2seed-May2015XX.abin
here is screen shot with log file, taxonomy and KEGG window. Please help rectify the problem