Hi Daniel, first of all, thanks for your work with Megan. With 6.18.0, I do the following:
- generate diamond daa file (blastx fastq to diamond nr DB, here 2 sample lines from diamond view -a):
m181204_201404_42243_c101474132550000001823318602141954_s1_p0/394/ccs gi|489855549|ref|WP_003759213.1| 79.5 112 20 1 921 1256 17 125 1.6e-40 174.5
m181204_201404_42243_c101474132550000001823318602141954_s1_p0/394/ccs gi|394329057|gb|EJE55184.1| 82.0 100 18 0 951256 1 100 3.3e-38 166.8
- meganize DAA file (file -> meganize DAA file -> select file to meganize)
- select the daa file above and load the megan-map-Oct2019.db as database (taxonomy -> loadMeganMapDB mapping file)
In the end, not a single sequence is assigned. Any ideas what I am missing? Any help appreciated.