I am currently trying to identify MAGs using MEGAN6 and diamond. I generated blast tabular files using diamond and the up to date ncbi nr prot db and this appears to have worked properly producing lines like:
“megahit_10_000000391325 KRO36477.1 87.8 337 41 0 1013 3 1 337 1.2e-178 636.0”
When I load a MAG into MEGAN using “import blast” and then load the “prot_acc2tax-Nov2016.abin” as a mapping file all reads are unassigned.
Is there a fix for this? Should I be altering some internal setting?
I am currently using the standard settings throughout MEGAN6.