Problem importing BLAST files


#1

Hi I found problems importing a BLAST file in txt format with MEGAN6. The message is: Execute failed: java.io. IOException. File not a BLASTN file in text format. The stange is that the same file is imported by MEGAN 5
thanks
Pasquy


#2

Please send me the file…


#3

blast output_newdb_format8_FS-89_olea_filter_out.txt.zip (2.8 MB)

Here you have. Thanks Pasquale. Uploading…


#4

The file imports just fine.
Please make sure that you set the “Format” to BlastTab and the “Mode” to BlastN in the Import from Blast dialog. At present MEGAN wrongly detects the “Format” as RDP, which is incorrect.


#5

Thanks a lot Daniel. It works
Pasquale


#6

Dear Daniel,
sorry again. It seems to work but unfortunately, when I upload the file the contigs are al unclassified
Pasquale


#7

Are you sure that you used the mapping file nucl_acc2tax-Mar2018.abin for taxonomic mapping?
Available here:
http://ab.inf.uni-tuebingen.de/data/software/megan6/download/nucl_acc2tax-Mar2018.abin.zip