Hi MEGAN community,
I am relatively new to MEGAN. So far I meganized diamond daa files and want to analysis the data in graphical interface. I used compare function for all the samples. For the first step I want to check the percentage of the unassigned reads in the taxonomy or functional annotation for each sample. For that I used export…text csv format… taxonname to count … summarized…
However, I realized for each sample if I sum manually all the counts(that is also number of reads, right?)of all subnodes of a given node, it does not equal to the count of the given node. For examples,
cellular organisms != Bacteria+Eukaryota
NCBI != Bacteria+Eukaryota+Viruses+Not assigned
Why is that? Did I miss something?