Dear MEGAN community,
For some reason, MALT does not want to assign a particular taxon (Anaerolineacea sp oral taxon 439). I run my data through MALT, and open it in MEGAN to find all the reads that should be assigned to this taxon in the “not assigned” bin. Upon inspecting the alignments, they pass my desired LCA parameters, but are somehow not assigned:
I can fix this by recalculating LCA parameters in MEGAN, but I have >500 samples, and don’t want to have to do this separately for each file…
Any help would be greatly appreciated.